Journal: Aging Cell

Article Title: Diminished and Altered Cellular Senescence Response in Delayed Wound Healing of Aging

doi: 10.1111/acel.70493

Figure Lengend Snippet: Transient upregulation of SA‐β‐gal activity, p16, and 21‐positive cells during wound healing in young but not in old mice. (A) Representative immunofluorescence images of wound tissue sections from young mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue) to visualize nuclei. Epi = epidermis, der = dermis for reference. (B) Representative immunofluorescence images of wound tissue sections from old mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue). (C) Quantification of the percentage of SA‐β‐gal‐positive cells in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (D) Quantification of p16 mRNA relative expression in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (E, F) Representative immunofluorescence images demonstrating p21 (red) and DAPI (blue) staining in wound tissue from young (E) and aged (F) mice at 0, 6, 12, 18, and 24 days postwounding. The epidermis (epi) and dermis (der) are indicated for reference. (G) Quantification of the percentage of p21‐positive cells in wound tissue from young and old mice over the wound healing time course. (H) qRT‐PCR analysis of p21 gene expression in wound tissue from young and aged mice over the wound healing time course, shown as relative expression to β‐Actin. N = 5 per age group per time point. * p < 0.05, ** p < 0.01.

Article Snippet: Nuclei were counterstained with Akoya Spectra DAPI (FP1490, Akoya Biosciences), and slides were mounted with ProLong Gold Antifade Mountant ( P36930 , Invitrogen, Waltham, MA, USA).

Techniques: Activity Assay, Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Gene Expression